Introduction: Few studies were conducted to determine the effects of lighting on cognitive performance. However, they could not reach a decisive conclusion. This study investigated cognitive performance of university students exposed to different levels of lighting under laboratory conditions.

Methods: In this experimental study, 33 subjects (age range 19-26 years) performed cognitive tests. Participants were exposed to three levels of lighting (200, 500 and 1500lux) in laboratory conditions while performing CPT tests to investigate percentage of attention and reaction time machine that measures reaction time.

Results: The results of this study showed that the maximum percentage of attention (99.75%) belonged to lighting of 1500 (lux) and the minimum percentage of attention was related to 500(lux) (99.36%). statistical analysis showed significant differences in percentage of attention in different levels of lighting (P=0.004). In addition, results of data analysis showed that increase in intensity of  lighting can make a significant change in the average response time (P˂0.001), correct response (P=0.004), commission error (P=0.001) and omission error (P=0.017). With increasing the lighting intensity, reaction time has decreased. The reaction time showed significant differences  at all levels of lighting (P˂0.001)

Conclusion: According to the findings of this study, lighting causes a decrease in reaction time and increase in attention. Thus, the lighting should be taken into account while designing of job and tasks which need attention or reaction time.